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murine macrophage cell line raw264 7  (ATCC)


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    ATCC murine macrophage cell line raw264 7
    Murine Macrophage Cell Line Raw264 7, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+raw264+7+cell+line/RAW+264%2E7/pmc13059125-142-1-6
    Average 99 stars, based on 24281 article reviews
    murine macrophage cell line raw264 7 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Potency Assay:

    Article Title: HC-HA/PTX3 from amniotic membrane reprograms human corneal fibroblasts to neural crest progenitors by switching from canonical to noncanonical TGFβ signaling
    Article Snippet: .. Finally, each batch of HC-HA/PTX3 was released after passing a potency assay by exhibiting no less than (NLT) 90% of inhibiting the tartrate-resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC, Manassas, VA) promoted by receptor activator of nuclear factor kappa-Β ligand (RANKL) [ ]. ..

    Article Title: HC-HA/PTX3 from amniotic membrane reprograms human corneal fibroblasts to neural crest progenitors by switching from canonical to noncanonical TGFβ signaling.
    Article Snippet: .. Finally, each batch of HC-HA/PTX3 was released after passing a potency assay by exhibiting no less than (NLT) 90% of inhibiting the tartrate-resistant acid phosphatase (TRAP) ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC, Manassas, VA) promoted by receptor activator of nuclear factor kappa-Β ligand (RANKL) (40). ..

    Article Title: HC-HA/PTX3 from amniotic membrane reprograms human corneal fibroblasts to neural crest progenitors by switching from canonical to noncanonical TGFβ signaling.
    Article Snippet: .. In addition, each batch of HC-HA/PTX3 was released after passing the potency assay with the acceptance criteria of no less than 89% inhibition of tartrate-resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC; Manassas, VA) by receptor activator of nuclear factor kappa-Β ligand (PeproTech; Cranbury, NJ). ..

    Article Title: Human birth tissue products as a non-opioid medicine to inhibit post-surgical pain
    Article Snippet: It was released by confirming the identity of HC- HA/PTX3 based on Western blot analysis using respective antibody specific to HC1 (ITIH1 antibody, Cat# ab70048, Abcam, Waltham, MA, USA) and PTX3 (PTX3 antibody, Cat# ALX- 804- 464- C100, Enzo, Farmingdale, NY, USA) with or without hyaluronidase (HAase) digestion to release HC1 and HMW PTX3 from HC- HA/PTX3 in the loading well into the gel and with or without reduction by DTT, which further rendered PTX3 from HMW (octamer) to dimer and monomer (Figure 3—figure supplement 1C, D). .. In addition, each batch of HC- HA/PTX3 was released after it also passed the potency assay (Figure 3—figure supplement 1E), with the acceptance criterion of no less than 89.21% inhibition of tartrate- resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC, Manassas, VA, USA) by receptor activator of nuclear factor kappa-Β ligand (RANKL) (PeproTech, Cranbury, NJ, USA). .. Lumbar DRGs from 4- week- old WT mice and CD44 KO mice (both sexes) were collected in cold DH10 (Weng et al., 2015) (90% Dulbecco's Modified Eagle Medium [DMEM]/F- 12, 10% fetal bovine serum, penicillin [100 U/ml], and streptomycin [100 μg/ml] [Invitrogen]) and treated with enzyme solution Zhang, Huang, Ford et al. eLife 2024;13:RP101269.

    Article Title: Human birth tissue products as a non-opioid medicine to inhibit post-surgical pain
    Article Snippet: It was released by confirming the identity of HC-HA/PTX3 based on Western blot analysis using respective antibody specific to HC1 (ITIH1 antibody, Cat# ab70048, Abcam, Waltham, MA, USA) and PTX3 (PTX3 antibody, Cat# ALX-804-464-C100, Enzo, Farmingdale, NY, USA) with or without hyaluronidase (HAase) digestion to release HC1 and HMW PTX3 from HC-HA/PTX3 in the loading well into the gel and with or without reduction by DTT, which further rendered PTX3 from HMW (octamer) to dimer and monomer ( ). .. In addition, each batch of HC-HA/PTX3 was released after it also passed the potency assay , with the acceptance criterion of no less than 89.21% inhibition of tartrate-resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC, Manassas, VA, USA) by receptor activator of nuclear factor kappa-Β ligand (RANKL) (PeproTech, Cranbury, NJ, USA). .. Lumbar DRGs from 4-week-old WT mice and CD44 KO mice (both sexes) were collected in cold DH10 ( ) (90% Dulbecco's Modified Eagle Medium [DMEM]/F-12, 10% fetal bovine serum, penicillin [100 U/ml], and streptomycin [100 μg/ml] [Invitrogen]) and treated with enzyme solution (dispase [5 mg/ml] and collagenase type I [1 mg/ml] in Hanks’ balanced salt solution without Ca 2+ or Mg 2+ [Invitrogen]) for 35 min at 37°C.

    Activity Assay:

    Article Title: HC-HA/PTX3 from amniotic membrane reprograms human corneal fibroblasts to neural crest progenitors by switching from canonical to noncanonical TGFβ signaling
    Article Snippet: .. Finally, each batch of HC-HA/PTX3 was released after passing a potency assay by exhibiting no less than (NLT) 90% of inhibiting the tartrate-resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC, Manassas, VA) promoted by receptor activator of nuclear factor kappa-Β ligand (RANKL) [ ]. ..

    Article Title: HC-HA/PTX3 from amniotic membrane reprograms human corneal fibroblasts to neural crest progenitors by switching from canonical to noncanonical TGFβ signaling.
    Article Snippet: .. Finally, each batch of HC-HA/PTX3 was released after passing a potency assay by exhibiting no less than (NLT) 90% of inhibiting the tartrate-resistant acid phosphatase (TRAP) ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC, Manassas, VA) promoted by receptor activator of nuclear factor kappa-Β ligand (RANKL) (40). ..

    Article Title: HC-HA/PTX3 from amniotic membrane reprograms human corneal fibroblasts to neural crest progenitors by switching from canonical to noncanonical TGFβ signaling.
    Article Snippet: .. In addition, each batch of HC-HA/PTX3 was released after passing the potency assay with the acceptance criteria of no less than 89% inhibition of tartrate-resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC; Manassas, VA) by receptor activator of nuclear factor kappa-Β ligand (PeproTech; Cranbury, NJ). ..

    Article Title: Human birth tissue products as a non-opioid medicine to inhibit post-surgical pain
    Article Snippet: It was released by confirming the identity of HC- HA/PTX3 based on Western blot analysis using respective antibody specific to HC1 (ITIH1 antibody, Cat# ab70048, Abcam, Waltham, MA, USA) and PTX3 (PTX3 antibody, Cat# ALX- 804- 464- C100, Enzo, Farmingdale, NY, USA) with or without hyaluronidase (HAase) digestion to release HC1 and HMW PTX3 from HC- HA/PTX3 in the loading well into the gel and with or without reduction by DTT, which further rendered PTX3 from HMW (octamer) to dimer and monomer (Figure 3—figure supplement 1C, D). .. In addition, each batch of HC- HA/PTX3 was released after it also passed the potency assay (Figure 3—figure supplement 1E), with the acceptance criterion of no less than 89.21% inhibition of tartrate- resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC, Manassas, VA, USA) by receptor activator of nuclear factor kappa-Β ligand (RANKL) (PeproTech, Cranbury, NJ, USA). .. Lumbar DRGs from 4- week- old WT mice and CD44 KO mice (both sexes) were collected in cold DH10 (Weng et al., 2015) (90% Dulbecco's Modified Eagle Medium [DMEM]/F- 12, 10% fetal bovine serum, penicillin [100 U/ml], and streptomycin [100 μg/ml] [Invitrogen]) and treated with enzyme solution Zhang, Huang, Ford et al. eLife 2024;13:RP101269.

    Article Title: Human birth tissue products as a non-opioid medicine to inhibit post-surgical pain
    Article Snippet: It was released by confirming the identity of HC-HA/PTX3 based on Western blot analysis using respective antibody specific to HC1 (ITIH1 antibody, Cat# ab70048, Abcam, Waltham, MA, USA) and PTX3 (PTX3 antibody, Cat# ALX-804-464-C100, Enzo, Farmingdale, NY, USA) with or without hyaluronidase (HAase) digestion to release HC1 and HMW PTX3 from HC-HA/PTX3 in the loading well into the gel and with or without reduction by DTT, which further rendered PTX3 from HMW (octamer) to dimer and monomer ( ). .. In addition, each batch of HC-HA/PTX3 was released after it also passed the potency assay , with the acceptance criterion of no less than 89.21% inhibition of tartrate-resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC, Manassas, VA, USA) by receptor activator of nuclear factor kappa-Β ligand (RANKL) (PeproTech, Cranbury, NJ, USA). .. Lumbar DRGs from 4-week-old WT mice and CD44 KO mice (both sexes) were collected in cold DH10 ( ) (90% Dulbecco's Modified Eagle Medium [DMEM]/F-12, 10% fetal bovine serum, penicillin [100 U/ml], and streptomycin [100 μg/ml] [Invitrogen]) and treated with enzyme solution (dispase [5 mg/ml] and collagenase type I [1 mg/ml] in Hanks’ balanced salt solution without Ca 2+ or Mg 2+ [Invitrogen]) for 35 min at 37°C.

    Clone Assay:

    Article Title: HC-HA/PTX3 from amniotic membrane reprograms human corneal fibroblasts to neural crest progenitors by switching from canonical to noncanonical TGFβ signaling
    Article Snippet: .. Finally, each batch of HC-HA/PTX3 was released after passing a potency assay by exhibiting no less than (NLT) 90% of inhibiting the tartrate-resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC, Manassas, VA) promoted by receptor activator of nuclear factor kappa-Β ligand (RANKL) [ ]. ..

    Article Title: HC-HA/PTX3 from amniotic membrane reprograms human corneal fibroblasts to neural crest progenitors by switching from canonical to noncanonical TGFβ signaling.
    Article Snippet: .. Finally, each batch of HC-HA/PTX3 was released after passing a potency assay by exhibiting no less than (NLT) 90% of inhibiting the tartrate-resistant acid phosphatase (TRAP) ACCEPTED MANUSCRIPT AR TIC LE IN PR ES S ARTICLE IN PRESS activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC, Manassas, VA) promoted by receptor activator of nuclear factor kappa-Β ligand (RANKL) (40). ..

    Article Title: HC-HA/PTX3 from amniotic membrane reprograms human corneal fibroblasts to neural crest progenitors by switching from canonical to noncanonical TGFβ signaling.
    Article Snippet: .. In addition, each batch of HC-HA/PTX3 was released after passing the potency assay with the acceptance criteria of no less than 89% inhibition of tartrate-resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC; Manassas, VA) by receptor activator of nuclear factor kappa-Β ligand (PeproTech; Cranbury, NJ). ..

    Article Title: Human birth tissue products as a non-opioid medicine to inhibit post-surgical pain
    Article Snippet: It was released by confirming the identity of HC- HA/PTX3 based on Western blot analysis using respective antibody specific to HC1 (ITIH1 antibody, Cat# ab70048, Abcam, Waltham, MA, USA) and PTX3 (PTX3 antibody, Cat# ALX- 804- 464- C100, Enzo, Farmingdale, NY, USA) with or without hyaluronidase (HAase) digestion to release HC1 and HMW PTX3 from HC- HA/PTX3 in the loading well into the gel and with or without reduction by DTT, which further rendered PTX3 from HMW (octamer) to dimer and monomer (Figure 3—figure supplement 1C, D). .. In addition, each batch of HC- HA/PTX3 was released after it also passed the potency assay (Figure 3—figure supplement 1E), with the acceptance criterion of no less than 89.21% inhibition of tartrate- resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC, Manassas, VA, USA) by receptor activator of nuclear factor kappa-Β ligand (RANKL) (PeproTech, Cranbury, NJ, USA). .. Lumbar DRGs from 4- week- old WT mice and CD44 KO mice (both sexes) were collected in cold DH10 (Weng et al., 2015) (90% Dulbecco's Modified Eagle Medium [DMEM]/F- 12, 10% fetal bovine serum, penicillin [100 U/ml], and streptomycin [100 μg/ml] [Invitrogen]) and treated with enzyme solution Zhang, Huang, Ford et al. eLife 2024;13:RP101269.

    Article Title: Human birth tissue products as a non-opioid medicine to inhibit post-surgical pain
    Article Snippet: It was released by confirming the identity of HC-HA/PTX3 based on Western blot analysis using respective antibody specific to HC1 (ITIH1 antibody, Cat# ab70048, Abcam, Waltham, MA, USA) and PTX3 (PTX3 antibody, Cat# ALX-804-464-C100, Enzo, Farmingdale, NY, USA) with or without hyaluronidase (HAase) digestion to release HC1 and HMW PTX3 from HC-HA/PTX3 in the loading well into the gel and with or without reduction by DTT, which further rendered PTX3 from HMW (octamer) to dimer and monomer ( ). .. In addition, each batch of HC-HA/PTX3 was released after it also passed the potency assay , with the acceptance criterion of no less than 89.21% inhibition of tartrate-resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC, Manassas, VA, USA) by receptor activator of nuclear factor kappa-Β ligand (RANKL) (PeproTech, Cranbury, NJ, USA). .. Lumbar DRGs from 4-week-old WT mice and CD44 KO mice (both sexes) were collected in cold DH10 ( ) (90% Dulbecco's Modified Eagle Medium [DMEM]/F-12, 10% fetal bovine serum, penicillin [100 U/ml], and streptomycin [100 μg/ml] [Invitrogen]) and treated with enzyme solution (dispase [5 mg/ml] and collagenase type I [1 mg/ml] in Hanks’ balanced salt solution without Ca 2+ or Mg 2+ [Invitrogen]) for 35 min at 37°C.

    Inhibition:

    Article Title: HC-HA/PTX3 from amniotic membrane reprograms human corneal fibroblasts to neural crest progenitors by switching from canonical to noncanonical TGFβ signaling.
    Article Snippet: .. In addition, each batch of HC-HA/PTX3 was released after passing the potency assay with the acceptance criteria of no less than 89% inhibition of tartrate-resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC; Manassas, VA) by receptor activator of nuclear factor kappa-Β ligand (PeproTech; Cranbury, NJ). ..

    Article Title: Human birth tissue products as a non-opioid medicine to inhibit post-surgical pain
    Article Snippet: It was released by confirming the identity of HC- HA/PTX3 based on Western blot analysis using respective antibody specific to HC1 (ITIH1 antibody, Cat# ab70048, Abcam, Waltham, MA, USA) and PTX3 (PTX3 antibody, Cat# ALX- 804- 464- C100, Enzo, Farmingdale, NY, USA) with or without hyaluronidase (HAase) digestion to release HC1 and HMW PTX3 from HC- HA/PTX3 in the loading well into the gel and with or without reduction by DTT, which further rendered PTX3 from HMW (octamer) to dimer and monomer (Figure 3—figure supplement 1C, D). .. In addition, each batch of HC- HA/PTX3 was released after it also passed the potency assay (Figure 3—figure supplement 1E), with the acceptance criterion of no less than 89.21% inhibition of tartrate- resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC, Manassas, VA, USA) by receptor activator of nuclear factor kappa-Β ligand (RANKL) (PeproTech, Cranbury, NJ, USA). .. Lumbar DRGs from 4- week- old WT mice and CD44 KO mice (both sexes) were collected in cold DH10 (Weng et al., 2015) (90% Dulbecco's Modified Eagle Medium [DMEM]/F- 12, 10% fetal bovine serum, penicillin [100 U/ml], and streptomycin [100 μg/ml] [Invitrogen]) and treated with enzyme solution Zhang, Huang, Ford et al. eLife 2024;13:RP101269.

    Article Title: Human birth tissue products as a non-opioid medicine to inhibit post-surgical pain
    Article Snippet: It was released by confirming the identity of HC-HA/PTX3 based on Western blot analysis using respective antibody specific to HC1 (ITIH1 antibody, Cat# ab70048, Abcam, Waltham, MA, USA) and PTX3 (PTX3 antibody, Cat# ALX-804-464-C100, Enzo, Farmingdale, NY, USA) with or without hyaluronidase (HAase) digestion to release HC1 and HMW PTX3 from HC-HA/PTX3 in the loading well into the gel and with or without reduction by DTT, which further rendered PTX3 from HMW (octamer) to dimer and monomer ( ). .. In addition, each batch of HC-HA/PTX3 was released after it also passed the potency assay , with the acceptance criterion of no less than 89.21% inhibition of tartrate-resistant acid phosphatase (TRAP) activity of osteoclast differentiation in cloned monocytes of murine RAW264.7 cell line (ATCC, Manassas, VA, USA) by receptor activator of nuclear factor kappa-Β ligand (RANKL) (PeproTech, Cranbury, NJ, USA). .. Lumbar DRGs from 4-week-old WT mice and CD44 KO mice (both sexes) were collected in cold DH10 ( ) (90% Dulbecco's Modified Eagle Medium [DMEM]/F-12, 10% fetal bovine serum, penicillin [100 U/ml], and streptomycin [100 μg/ml] [Invitrogen]) and treated with enzyme solution (dispase [5 mg/ml] and collagenase type I [1 mg/ml] in Hanks’ balanced salt solution without Ca 2+ or Mg 2+ [Invitrogen]) for 35 min at 37°C.

    Cell Culture:

    Article Title: Precise Control of the in vivo Fate of Nanomicelles Efficiently Treats Advanced Rheumatoid Arthritis via EGFR/JNK/MMP9 Pathway
    Article Snippet: Fluorescence Spectra The fluorescence spectra of FRET micelles were measured using a fluorescence spectrometer (Lambda 365, PerkinElmer, USA) at an excitation wavelength of 640 nm with a slit width of 2 nm, integration time of 0.1 s, and increment of 0.5 nm. .. Emission spectra were collected from 500 to 900 nm. https://doi.org/10.2147/IJN.S511421 International Journal of Nanomedicine 2025:20 5356 Powered by TCPDF (www.tcpdf.org) Cell Culture and Treatments The murine RAW264.7 cell line was purchased from the American Type Culture Collection (ATCC, USA). .. RAW264.7 cells were cultured with Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (GIBCO, USA) and 1% penicillin–streptomycin solution in an incubator with 5% CO2 at 37 °C.



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    Preparation and characterization of BM@MnO 2 @AB680 nanoparticles. (a) The TEM image of SiO 2 @MnO 2 nanoparticles. (b) The TEM image of hollow MnO 2 (MnO 2 ) nanoparticles. (c) The TEM image of hollow MnO 2 loaded with AB680 (MnO 2 @AB680) nanoparticles. (d) The TEM image of bacterial membrane (BM)-coated hollow MnO 2 loaded with AB680 (BM@MnO 2 @AB680) nanoparticles. (e) The particle sizes of different MnO 2 based nanoparticles. (f) The zeta potentials of BM and different nanoparticles. (g) The PDI of different MnO 2 based nanoparticles. (h) The particle size stability of BM@MnO 2 @AB680 nanoparticles. (i) The zeta potential stability of BM@MnO 2 @AB680 nanoparticles. (j) The PDI stability of BM@MnO 2 @AB680 nanoparticles. (k) Relative oxygen level in different groups within PBS added with H 2 O 2 . (l) CLSM images of RAW264.7 cells incubated with Cy5.5 labeled MnO 2 @AB680 (left) and BM@MnO 2 @AB680 (right) nanoparticles. Scale bar: 100 μm. (m) Mean fluorescence intensity (MFI) of RAW264.7 cells after incubation with MnO 2 @AB680 (Mac@AMn) and BM@MnO 2 @AB680 (Mac@ABMn) nanoparticles. (n) MFI of RAW264.7 cells after incubation with BM@MnO 2 @AB680 with different time.

    Journal: Materials Today Bio

    Article Title: Nano-bionic bacteria armed adoptive macrophage therapy against bladder cancer

    doi: 10.1016/j.mtbio.2026.103130

    Figure Lengend Snippet: Preparation and characterization of BM@MnO 2 @AB680 nanoparticles. (a) The TEM image of SiO 2 @MnO 2 nanoparticles. (b) The TEM image of hollow MnO 2 (MnO 2 ) nanoparticles. (c) The TEM image of hollow MnO 2 loaded with AB680 (MnO 2 @AB680) nanoparticles. (d) The TEM image of bacterial membrane (BM)-coated hollow MnO 2 loaded with AB680 (BM@MnO 2 @AB680) nanoparticles. (e) The particle sizes of different MnO 2 based nanoparticles. (f) The zeta potentials of BM and different nanoparticles. (g) The PDI of different MnO 2 based nanoparticles. (h) The particle size stability of BM@MnO 2 @AB680 nanoparticles. (i) The zeta potential stability of BM@MnO 2 @AB680 nanoparticles. (j) The PDI stability of BM@MnO 2 @AB680 nanoparticles. (k) Relative oxygen level in different groups within PBS added with H 2 O 2 . (l) CLSM images of RAW264.7 cells incubated with Cy5.5 labeled MnO 2 @AB680 (left) and BM@MnO 2 @AB680 (right) nanoparticles. Scale bar: 100 μm. (m) Mean fluorescence intensity (MFI) of RAW264.7 cells after incubation with MnO 2 @AB680 (Mac@AMn) and BM@MnO 2 @AB680 (Mac@ABMn) nanoparticles. (n) MFI of RAW264.7 cells after incubation with BM@MnO 2 @AB680 with different time.

    Article Snippet: RAW264.7 murine macrophage cell lines were purchased from ATCC, which were cultured in Dulbecco's modified Eagle's medium (DMEM) -high glucose medium.

    Techniques: Membrane, Zeta Potential Analyzer, Incubation, Labeling, Fluorescence

    AN and FV reduce inflammatory NO production in LPS-challenged RAW264.7 cells. RAW264.7 cells were co-treated with LPS (250 ng/mL) and AN, FV, or quercetin (20 µM) for 24 h. Nitrite levels in culture supernatants were quantified using the Griess Reagent System. Absorbance was measured at 548 nm, and nitrite concentrations were normalized to the LPS control. The dotted line at 100% indicates the normalized LPS control and serves as a visual reference baseline for comparison between groups. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test. Data are presented as median with interquartile range (IQR), whiskers indicating minimum and maximum values, and individual data points from three independent experiments ( n = 9). Statistical significance is indicated as ** p ≤ 0.01 and **** p ≤ 0.0001.

    Journal: Marine Drugs

    Article Title: From Sea to Cell: Ascophyllum nodosum and Fucus vesiculosus Extracts Attenuate NF-κB-Mediated Inflammation and Protect Intestinal Barrier Integrity—A Comprehensive Analysis Applying In Vitro and In Vivo Models

    doi: 10.3390/md24050182

    Figure Lengend Snippet: AN and FV reduce inflammatory NO production in LPS-challenged RAW264.7 cells. RAW264.7 cells were co-treated with LPS (250 ng/mL) and AN, FV, or quercetin (20 µM) for 24 h. Nitrite levels in culture supernatants were quantified using the Griess Reagent System. Absorbance was measured at 548 nm, and nitrite concentrations were normalized to the LPS control. The dotted line at 100% indicates the normalized LPS control and serves as a visual reference baseline for comparison between groups. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test. Data are presented as median with interquartile range (IQR), whiskers indicating minimum and maximum values, and individual data points from three independent experiments ( n = 9). Statistical significance is indicated as ** p ≤ 0.01 and **** p ≤ 0.0001.

    Article Snippet: The murine macrophage-like cell line RAW264.7 (ATCC, Manassas, VA, USA) and the human monocytic cell line THP-1 (DSMZ, Braunschweig, Germany) were maintained at 37 °C in a humidified atmosphere containing 5% CO 2 .

    Techniques: Control, Comparison

    The effects of H. triquetrifolium on ( A ) PGE 2 levels and ( B ) IL-6 levels in LPS-stimulated RAW264.7 cells. The value of 0.5 mg/mL is the highest non-cytotoxic effective concentration. Control: control group with DMEM; LPS: control group stimulated with LPS only; LPS: lipopolysaccharide from E. coli ; IND: Indomethacin (100 μM); L-NAME: N(gamma)-nitro-L-arginine methyl ester; PGE 2 : prostaglandin E 2 ; IL-6: interleukin-6. Statistically significant differences for each compound are indicated against LPS (* p < 0.05).

    Journal: Molecules

    Article Title: In Vitro Evidence for the Dual Antioxidant and Anti-Inflammatory Roles of Hypericum triquetrifolium in Cancer Therapy: Selective Cytotoxicity Against Pancreatic Cancer Cells

    doi: 10.3390/molecules31101628

    Figure Lengend Snippet: The effects of H. triquetrifolium on ( A ) PGE 2 levels and ( B ) IL-6 levels in LPS-stimulated RAW264.7 cells. The value of 0.5 mg/mL is the highest non-cytotoxic effective concentration. Control: control group with DMEM; LPS: control group stimulated with LPS only; LPS: lipopolysaccharide from E. coli ; IND: Indomethacin (100 μM); L-NAME: N(gamma)-nitro-L-arginine methyl ester; PGE 2 : prostaglandin E 2 ; IL-6: interleukin-6. Statistically significant differences for each compound are indicated against LPS (* p < 0.05).

    Article Snippet: The RAW264.7 murine macrophage cell line (ATCC, Manassas, VA, USA) was cultured in DMEM (Gibco, Paisley, UK) supplemented with 10% FBS, streptomycin (10,000 μg/mL), and 1% penicillin (10,000 units/mL) in a humidified atmosphere at 37 °C with 5% CO 2 .

    Techniques: Concentration Assay, Control

    ROS analysis of RAW264.7 cells following H. triquetrifolium extract (HTE) treatment. ( A ) Representative immunocytochemistry images for ROS production in LPS-induced cells under indicated experimental conditions (Images captured via Cytell at 10× magnification). ( B ) Statistical analysis of fluorescence intensity. Fluorescence levels were quantified using ImageJ software, and statistical significance was determined via one-way ANOVA followed by Tukey’s post hoc test (GraphPad Prism). Data represent the mean ± SD; * p < 0.05 indicates statistical significance compared to the LPS-only group, **** p < 0.0001.

    Journal: Molecules

    Article Title: In Vitro Evidence for the Dual Antioxidant and Anti-Inflammatory Roles of Hypericum triquetrifolium in Cancer Therapy: Selective Cytotoxicity Against Pancreatic Cancer Cells

    doi: 10.3390/molecules31101628

    Figure Lengend Snippet: ROS analysis of RAW264.7 cells following H. triquetrifolium extract (HTE) treatment. ( A ) Representative immunocytochemistry images for ROS production in LPS-induced cells under indicated experimental conditions (Images captured via Cytell at 10× magnification). ( B ) Statistical analysis of fluorescence intensity. Fluorescence levels were quantified using ImageJ software, and statistical significance was determined via one-way ANOVA followed by Tukey’s post hoc test (GraphPad Prism). Data represent the mean ± SD; * p < 0.05 indicates statistical significance compared to the LPS-only group, **** p < 0.0001.

    Article Snippet: The RAW264.7 murine macrophage cell line (ATCC, Manassas, VA, USA) was cultured in DMEM (Gibco, Paisley, UK) supplemented with 10% FBS, streptomycin (10,000 μg/mL), and 1% penicillin (10,000 units/mL) in a humidified atmosphere at 37 °C with 5% CO 2 .

    Techniques: Immunocytochemistry, Fluorescence, Software